Expression of B Locus Beta 2 (<i>BLB2</i>) Gene at Cytolytic and Latent Immune Response Stages of Immunocompetence in Nigerian Indigenous Chickens

An economically-important trait in poultry for which gene identification continues to be a challenge is immune response. The objective of the study was quantitate expression major histocompatibility complex (MHC) class II BLB2 at cytolytic and latent response stages Nigerian indigenous chickens. A total 108 chickens (NIC) were sourced across South-western states Nigeria. The birds inoculated with sheep red blood cells (SRBC), after samples obtained (5 days post-inoculation) antibody haemagglutination test carried out place the birds into groups high low titre levels. categorisation resulted six normal feather high, normal low, naked neck high, frizzle frizzle feather groups. of 48 chicks selected from progeny expression studies. Surgical excision thymus spleen out for detection responses birds. β-actin used as endogenous control critical threshold method (2–ΔΔCт) was determination fold change. change spleen tissue 30,362.44 compared to 294.07; cytolytic immune 51.98 compared 5.24. At both of immune SRBC antigen, comparatively higher than height of transcriptional activity associated with stage. cytolytic and had mRNA expression. This concluded that Nigerian indigenous chicken induced stage repressed latent stage. During avian infections, category would perform better counterpart; protective response that offers will be repressed one time point other.